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Plant Materials |
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- Lotus japonicus MG-20 (Miyakojima)
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EMS treatment |
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- Rub seed's surface with sandpaper → absorption of water → add seeds in 0.4% (v/v) EMS solution → over night → wash seeds for 6 to 8 hrs → saw seeds on the peat moss.
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Growth Condition |
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- 23℃ 16h(light):8h(dark)
- After screening, seedlings are planted on soil (5cm in diameter).
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Medium |
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- 1/2 Okada and Shimura medium with 1.5% agar (OS medium : Aust J Plant Physiol 1992: 19, 439-448).
- Peat moss (Sakata seeds co., Japan)
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Screening on the agar medium |
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- Observing the form of one- to two-weeks-old seedlings on the 1/2 OS medium with 1.5% agar.
- About 20 seedlings were observed for each M1 line.
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Screening on the peat moss |
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- Observing the form of two- to three-weeks-old seedling.
- About 30 seedlings were observed for each M1 line.
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Observing the form of the adult phenotype |
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- After observations on the agar medium and the peat moss, one to three seeding were transplanted on soil.
- Observing the phenotype of two to three months old plants.
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Screening on the agar medium |
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Screening on the peat moss |
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Growth with the AraSystem |
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